Ethylene Glycolbis(sulfosuccinimidylsuccinate)
Properties
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Information about Ethylene Glycolbis(sulfosuccinimidylsuccinate) / CAS 167410-92-6
Ethylene Glycol Bis(sulfosuccinimidylsuccinate) (Sulfo-EGS) is a crosslinking agent used in biological research to study peptides and proteins. Each end of the Sulfo EGS molecule contains Sulfo-NHS esters (N-hydroxysuccinimide esters with a sulfonate group), which react with primary amine groups on polypeptide chains and the side-chain of lysine amino acids. The two NHS esters are separated by a 12-atom spacer arm.
Sulfo-EGS is water-soluble and membrane-impermeable, making it ideal for labeling the surfaces of cellular membranes. The crosslinks formed by Ethylene Glycol Bis(sulfosuccinimidylsuccinate) can be broken by reacting with hydroxylamine at a pH of 8.5.
A related compound, EGS (Ethylene Glycol Bis(succinimidylsuccinate)), is water-insoluble, which makes it membrane-permeable. EGS is used primarily for studying intracellular interactions.
References:
- Abdella, R.M., Smith, P.K. and Royer, G.P. (1979) Biochem. Biophys. Res. Comman. 87, 734-742.
Safety
- H315 Causes skin irritation.
- H319 Causes serious eye irritation.
- P280 Wear protective gloves/protective clothing/eye protection/face protection.
- P303+P361+P353 IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.
- P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.